Cartridge contents are produced in a multitude of ways, resulting in extracts that can contain many different ingredients, both cannabis-derived and not.
Vape cartridges are rapidly growing more popular with new cannabis consumers, and it’s not difficult to fathom why: They are portable, discreet and usually less pungent than flower. During the first four months of 2018, Californians purchased $165 million worth of vape carts, Coloradans shelled out $62.4 million for them and Oregonians spent $31 million, according to data from BDS Analytics, making cartridges the top-selling product in all three states. Given the hype, let’s examine both cartridges and their contents, as there is a wide range of quality on the market.
Cartridges
While there might be exceptions, cartridges (the vessels holding the cannabis extracts) can largely be categorized as high or low quality.
Typically, low-quality cartridges:
are made of plastic (terpenes can penetrate plastic, and plastic can potentially leach chemicals from the oil),
have poor-quality or ill-fitting O-rings that leak; and
have pre-moistened wicks primed with glycerin or propylene glycol that can cause allergic reactions in some people.
Low-quality cartridges will have a higher customer return rate (if a return policy exists) and will drive away customers who become frustrated with the lackluster experience.
High-quality cartridges typically:
are made of premium materials, such as glass, metal and ceramic;
have properly-sized O-rings; and
have sealed joints that prevent contact between the air and cartridge contents.
Choose your cartridges wisely and always examine the cartridge’s quality. A low-cost cartridge is not necessarily better for your business, and it alerts the customer that the contents might be poor-quality, too.
Contents
As consumers become more educated about their options, it is likely they will begin to examine your cartridge’s stated ingredients, the same as they do for food ingredients, ultimately affecting how dispensary purchasing managers approach you. Whether you are vertically integrated or working with a third-party extractor, it’s crucial you know everything about your product. Do you claim to use organic practices or to be chemical free? Do you have certifications proving it? Does your product contain cannabis-derived terpenes, artificial flavors or terpenes derived from other sources? What terpene-isolation method was utilized? If non-cannabis-derived terpenes or artificial flavors were used, what are they, and from where were they sourced? If a purchasing manager asks a question about your product that you cannot answer, you’re in trouble.
Here is a rundown of contents found in typical vape cartridges:
1. Cannabis-derived terpenes: Cannabis terpenes sourced from cannabis.
Full-spectrum in composition, products made with these terpenes contain a high percentage of monoterpenes that have not been oxidized or degraded by heat application.
2. Steam-distilled terpenes: Softer in taste than extracted terpenes that have been isolated without utilizing heat, many steam-distilled terpenes are lost in the water used to produce steam, aka “pot water.”
3. Hydrosols: Hydrosols are a byproduct of steam distillation and low-heat distillations. They are classified as floral waters (i.e., essential oils) and contain only small percentages of actual terpenes. Heat is utilized and degrades the terpenes, too.
4. Non-cannabis-derived terpenes: Terpenes sourced directly from plant leaves, fruits or other organic sources, rather than from cannabis. It is impossible to recreate the aroma or flavor of the original plant/cultivar utilizing terpenes from non-cannabis plants, but a gross approximation can be achieved.
5. Artificial flavors: Typically, the artificial flavors found in cannabis cartridges are sourced from the e-cigarette industry. There are thousands of flavors, but their safety is in question (e.g., diacetyl causing “popcorn lung”).
6. HTFSE (High-Terpene Full-Spectrum Extract): Made from hydrocarbon extraction, there has been a recent trend of producing these products from pressed rosin. Also called sauce, HTFSE has high terpene content and is aromatic and flavorful.
7. CO2 Extracted: Some CO2 extractors collect a few available terpenes from CO2 extraction, but, more often than not, the cannabis product utilized to extract is dried, thus much of the available monoterpenes are lost in the drying process. This will result in a terpene composition that is mostly comprised of basic primary terpenes and low percentages of available monoterpenes. Therefore, both the final aroma and flavor are not as strong as HTFSEs, or if you had utilized a no-heat methodology of terpene isolation.
Beyond customers and purchasing managers, an important production-related detail to keep in mind is whether the stated THC percentage is measured before or after viscosity adjustment (fine-tuning the oil’s density) with glycerin, glycol and hydrosols. If any of these products were added after lab testing, the stated THC percentage is higher than what the product actually contains, making the stated percentage erroneous and potentially opening you to a lawsuit.
Most quality cartridges contain either CO2, hydrocarbon or distilled extracts, or a combination thereof, and most have flavor added. Some add cannabis-derived terpenes to a distillate to approximate the original characteristics of the plant/cultivar from which it came. This is typically accomplished by adding a fresh-frozen, terpene-rich hydrocarbon extract to a distillate. The resulting extract is flavorful and has a preferred viscosity.
Some utilize steam-distilled cannabis terpenes and hydrosols (a type of floral water), but these often lack monoterpenes (e.g., geraniol, terpineol, limonene, myrcene, linalool, pinene, etc.), which are responsible for the differentiation between cultivars. Some companies claim to re-infuse cannabis terpenes in their products, but said terpenes are often manufactured via low-heat steam distillation (utilizing distilled water and ethanol, or a variation thereof). The oxygen- and water-exposure results in a product with few of the original terpenes.
sheilaf2002 | Adobe Stock
How to Decide
All extracts, isolates and compounds mentioned can be added to a flavorless or close-to-flavorless distillate or extract to increase aroma and flavor. This endless supply of cannabinoid cocktails has led to a great disparity in overall quality with respect to the desirable traits of the original cultivar. What looks low-quality and what is low-quality can be difficult to distinguish.
Some customers choose the clear oil over the dark oil, thinking it is purer and superior; this is generally a good rule of thumb to follow, as a dark oil typically indicates excessive amounts of lipids, fats, wax, or pigments in the product, or improper storage of extract material leading to exposure to air (oxidization), heat (decarboxylation) or a multitude of other factors.
That does not mean all darker colored extracts are inferior. Case in point: If one were to add a HTFSE to a water-clear distillate, it would inevitably add color. The more HTFSE added, the darker the distillate will become. Pressed rosin will impart undesired darker color when added to a clear distillate, yet the flavor profiles it imparts are strong when the rosin is produced at low temperatures (which preserves the available terpenes).
Cannabinoid content is another factor that can be misleading. If a distillate is made up of 95-percent cannabinoids, it contains 5-percent non-cannabinoid content, which can be terpenes, wax, pigments, flavonoids, etc. If a distillate is made up of 99-percent cannabinoids, it obviously has fewer of these non-cannabinoid compounds. While it might sound appealing to the unwitting consumer, a vape pen cartridge that contains 99-percent cannabinoids may not be pleasant to consume because terpenes are what add flavor and aroma.
Having the most potent cartridge at the expense of other desirable attributes may eventually work as a disadvantage for the cartridge producer. A vape cartridge should contain a perfect balance of both cannabinoids and terpenes. Within that larger scope, manufacturers can formulate specific cannabinoid and terpene ratios to cater to customer desires or requests. If a group of customers only wants CBD distillate at 80-percent cannabinoid, 20-percent terpene ratio, with no THC, you will be able to formulate that. If another desires an 80-percent THC cartridge combined with 20 percent terpenes, you can do that too.
Questions Ahead
As we develop new products and formulations within this space, we will also have to wrestle with health and safety concerns. I’ll be the first to admit that there are a lot of unknowns with cannabis. For starters: How much is too much? It’s a simple question, yet there is certainly no easy answer given the vast number of terpenes in cannabis and how they interact with different metabolisms, different body weights and a whole host of other factors that determine what thresholds of terpenes are healthy (or perhaps detrimental) to an individual.
There are even more unanswered questions regarding the medical applications of cannabinoids. What is it about the synergistic effects of cannabinoids, terpenes and the specific blending of the two that can produce the pharmaceuticals of the future? What combinations of cannabinoids and terpenes treat which types of cancer? What combination can be used as a neuroprotectant? Given the worldwide cancer rate, and the worldwide need for neuroprotectants for diseases such as Alzheimer’s and dementia, I believe there is great monetization incentive to develop these drugs.
David Bernard-Perron, VP of Cultivation Operations, TGOD
Photos By Amer Nabulsi
(11/8/2019) Editor's note: This story has been updated for clarity. While David Bernard-Perron's living soil recipe at Whistler Medical Marijuana Corp. (WMMC) was certified organic in 2014, WMMC received its first organic certification in 2013, the year before Bernard-Perron joined the company.
On paper, it’s hard to understand the scale at which The Green Organic Dutchman plans to operate. What does nearly 4 acres (or 1.5 hectares) of cannabis grown under a glass roof look like? What about just under 20 acres, which equates to roughly 8 hectares? How much soil does an organic farm of that size require? What precautions must be taken and what tools implemented to ensure a consistently healthy crop while still maintaining a living soil?
Seeing that scale in person is dizzying: row after row of mobile racks topped with custom-made pots filled with a house-made living soil—a mixture of rich earth, molasses, beneficial microbes, bacteria, fungi, insects, nematodes—masterminded by David Bernard-Perron, the company’s vice president of cultivation operations.
After obtaining his master’s in agriculture from McGill University in Montreal, Quebec, Canada, in 2015, Bernard-Perron moved to the Canadian West Coast and launched his career in the cannabis industry as the head agrologist for Whistler Medical Marijuana Corp., a licensed medical cannabis operation in British Columbia. There, he developed and refined the company’s indoor living soil program.
TGOD, as his current employer is known, hopes to replicate the success Bernard-Perron found in Whistler’s 15,000-square-foot operation, first with the company’s 160,000-square-foot Ancaster, Ontario, facility, then again in its 1.3 million-square-foot behemoth in Valleyfield, Quebec.
Getting to scale has definitely not been easy, and recent headlines about the company losing the funding it needed to complete construction at both sites certainly have complicated matters, but executives remain confident that the setbacks are setting the company up for an even better launch forward.
Second-Mover Advantage
The company headquarters—half a floor at a nondescript office building above a bank near the Toronto International Airport in Mississauga—belies the company’s vision: to be the world’s largest organic cannabis producer. The space doesn’t quite fit the team’s administrative and executive divisions, but no one complains; they know making that organic vision a reality requires time and sacrifice.
“It’s a culture of entrepreneurs that are here from various backgrounds, various industries to create something great that’s long-lasting,” says Drew Campbell, TGOD’s head of marketing. (The company’s headquarters isn’t a Silicon Valley garage, either, which also helps ease cramped tensions.)
Instead of office space, the bulk of the company’s funds have gone into completing construction of its hybrid glass-roof facilities, which, once completed, will officially make TGOD the world’s largest organic cannabis producer with a footprint of more than 1.4 million square feet in Canada alone.
That scale is the major differentiator between TGOD and any other producer in the world, says Brian Athaide, the company’s CEO. “There are ... other organic producers, but they tend to be more craft. We’re the only ones doing organic at scale that nobody’s ever done before.”
Building a facility with the automation and environmental control needed to produce a profitable crop at scale while maintaining organic standards was no easy task. TGOD went through several redesigns that delayed its market launch and increased costs. But Athaide says while those delays prevented the company from enjoying first-mover advantage, they allowed it to learn from others’ mistakes. For example, TGOD tripled its HVAC capacity after seeing reports of other licensed producers (LPs) struggling with humidity control. The Mississauga company also almost doubled the size of its processing facilities to accommodate the volume of flower it will be producing after seeing market bottlenecks in that area.
“So, we added more capital, we pushed back our timings, and I think that’s part of the … second-mover advantage because we were able to learn from everyone else,” Athaide says. “On the other hand, we haven’t really lost anything by not having that first-mover advantage on flower and oil because the rules around packaging are very strict. … So no one’s really built a brand of significance at this point yet.”
TGOD’s CBD Skunk Haze cultivar
Feed the Soil, Not the Plants
The company’s Valleyfield facility, its flagship, was still in the first construction phase before the company announced financial restructuring plans on Oct. 18. The Ancaster facility received final production approval from Health Canada on Oct. 16, allowing TGOD to move plants from the nursery/vegetation space into the hybrid greenhouses for full flowering. (TGOD had been using a few of those vegetation rooms to flower crops for its medical patients and to supply the Ontario Cannabis Store (OCS) with organic product.)
A room without plants, however, is the best way for visitors to grasp the intricacies of scaling organic cannabis. “Our facilities are purpose-built,” Athaide says.
Take the containers in which flowering plants will sit: Each room houses 1,450 pots filled with Bernard-Perron’s living soil blend. The pots had to be custom-made to fit perfectly in the company’s mobile tables, another custom-designed piece of equipment. Those two features combined allow TGOD to grow 5,800 plants in a single room, Bernard-Perron says.
The team took an unconventional approach to controlling how air circulates in those rooms. Instead of having fans above the canopy pushing air across the tops of its crops, “we have an air-vent system that will blow air from underneath and then that air moves through the canopy of the plant and then cools the leaf surface from underneath,” Bernard-Perron says. This system also allows the cultivation team to supplement with CO2 near the root zone and carry away excess moisture from deep within the canopy. “Then we exhaust it through the centralized air filtration system and then outside. So we get rid of the smell, and it’s the most efficient way to cool our product,” he adds.
Managing temperatures in a greenhouse with such a heat-sensitive crop is a significant challenge during Canadian summers, when outdoor temperatures can spike well above average to 100 degrees Fahrenheit. Operating at such a northern latitude also means TGOD needs supplemental lights to cultivate year-round. To solve the latter problem without exacerbating the former one, the company opted for LEDs. “Those lights are actually the closest spectrum we can find to natural sunlight,” Bernard-Perron explains, “and we have a better penetration inside a canopy, we use less energy, [so] we don’t have to cool as much to be able to have our target light level on our crop.” The company is studying how those fixtures affect the crops’ phytochemistry and yield and hopes to have results in 2020.
Workers spend their days scouting plants, part of TGOD’s IPM program.
More important than lighting is the company’s living soil program. A living soil, Bernard-Perron describes, is “soil in which we have brought back the natural process that you can find in nature. … We’re using a lot of beneficial microbes, bacteria, fungi, beneficial insects, nematodes, that all work together to transform the organic fertilizer we’re putting into the soil into living nutrients and to plant-available forms. So, we’re basically feeding the soil that then feeds the plants.”
“What the plants do in exchange for the soil is take CO2 from the air, use the sunlight to photosynthesize and transform that into sugar that they then push back into the soil,” Bernard-Perron says. “The plant is basically sweating sugar into the soil that feeds those beneficial micro-organisms. So, you have this awesome, built-in feedback loop that is your living soil system.”
In addition to lighting, the company is also studying how its organic cultivation approach impacts yields and plant chemistry. (Although more studies are needed, early data shows higher cannabinoid and terpene content, says Bernard-Perron.)
That soil’s microbiological diversity is also the company’s first line of defense in its integrated pest management (IPM) program. “Every handful of soil that we pick up [has] literally tens of thousands of kilometers of fungal network, fungal ivy, that are connecting the soil, the bacteria, and the plants and acting as a line of defense against other [predatory] micro-organisms,” Bernard-Perron says. “There’s so many good micro-organisms that are competing with everything and want to keep the plant healthy because the plants are giving them sugar. So they have to keep up their wall and keep pathogens outside.”
Four plants per container will sit under the custom Fluence LEDs in this room.
Organic Talk
Communicating these organic concepts to consumers is easier said than done, says TGOD’s head of marketing. But the slower market rollout gave the company more time to build its identity—or, as Campbell puts it, find its “story.” And that story is rooted in organics itself.
“Organic isn’t an adjective,” Campbell explains. “Organic is a fundamentally different consumer habit, [where] people now want to have transparency about where their products are coming from and what goes into [them].”
The Canadian market is fairly knowledgeable about what an organic certification means, and a large segment seeks out that certification in their produce, Athaide says. “Half of Canadian consumers buy something organic on a weekly basis, about 30% try to buy organic or kind of healthier products whenever they can,” he says. “[Public relations firm] Hill+Knowlton did a study earlier this year and found that 61% of medical patients and 50% of recreational consumers would prefer organic cannabis.”
While awaiting further test results on the effect the organic program has on TGOD’s yields and phytochemistry, education is focused on making the connection between cannabis and things people know: soil-grown, organic nutrients, no pesticides. That messaging gets out mainly through budtender education.
“We’ve launched a proprietary organic certification program for budtenders, educating [them] exactly on what is the difference between organic and non-organic growing,” Campbell says. “We want the first question for a budtender to ask when somebody goes in-store to be, ‘Would you prefer organic?’ If that is a starting point for our conversation, to make people aware that there is a difference between organic and non-organic cannabis, that’s a great differentiator for us and for a budtender to guide somebody towards our brand.”
TGOD chooses phenotypes that are easily processed at scale.
Sustainability-Driven
TGOD’s sustainability initiatives also play a big part in the company’s identity and messaging.
“Our tagline of ‘making life better’ might sound simple, but that’s really what we’re doing,” Campbell says. “On the consumer end, we’re delivering a certified organic product to individuals. … [But] making life better is, beyond just cannabis, how are we improving things? … Improving our legacy on our neighbors, our friends and our generations to come is something we take extremely seriously.”
Being as sustainable as possible permeates nearly every decision the company makes. For example, TGOD packaging is mostly glass. Not only is glass packaging 100% recyclable and avoids static cling (which can cause trichomes to stick to the sides of plastic containers), TGOD also uses its containers as marketing tools by posting videos on how to reuse them to grow herbs or repurpose them as organizational tools. In addition to the original content, that sustainability angle “gives us a bit of a leg up on some of the competition because we can talk about all those things that we are doing for the environment,” Campbell says.
TGOD maintains clean-room protocols in its entire facility.
Those environmental initiatives aren’t limited to containers. The company designed both of its facilities to be LEED-certified by the Canada Green Building Council pending finalization of construction and operations. LEED stands for leadership in energy and environmental design. It’s a widely known rating system for green buildings around the world, says Karine Cousineau, TGOD’s director of government relations and sustainability.
In addition to the sustainable materials used to build the facilities, the Ancaster site is decked with solar panels and a co-generation system that produces electricity to reduce the company’s load on the local power grid. The natural gas system also serves as a CO2 generator for the greenhouses and a heating source during the winter to keep the open-air rainwater basin from freezing. Any excess heat can also be shared with neighboring farms.
The TGOD team also collects an impressive amount of data, all processed by a centralized artificial intelligence system that will find efficiencies. Once fully built out, “there’ll be thousands, if not tens of thousands, of different sensors within these facilities that will track everything from external weather and environmental conditions to the movement of the plants internally to the soil conditions, the humidity, the lighting, and things that are happening down at a leaf level of a plant [through hyperspectral imagery],” says Geoff Riggs, TGOD’s chief information officer.
“All of that will be synthesized together into a very sophisticated data management platform, which then yields very novel and unique analytical insights,” he adds. It’s hard for Riggs to point to any one particular data set he’s eager to examine because, he says, “it’s not necessarily one set of data that’s most exciting—it’s the ability to combine all those different data sets to produce very interesting analytical observations.”
While some individuals and companies may look at sustainability from a purely environmental perspective, TGOD sees it as much more than that. “It’s also the whole social side of things and governance,” Cousineau says. To that end, she and her team are working on a survey “where basically we work with our stakeholders to determine: What should be the most important for TGOD? What should we track? What kind of KPIs [key performance indicators] should we put in place?” And at TGOD, stakeholder is a broadly inclusive term. The company not only sends surveys to management, “but also every stakeholder we have, from neighbors, employees, legislators, customers, consumers,” she continues, the goal being to have everyone “be part of what we’re building.”
Each cultivation room at the Ancaster facility holds 1,450 custom pots.
Striving for Long-Term Stability
Despite the company’s best efforts, slow retail licensing has dampened the legal market and kept the illicit market alive and well, according to Athaide. This caused market investors to pull investments across the board, sending cannabis stocks tumbling and shrinking the ability for cannabis companies to raise capital. TGOD announced a review of alternative financing options on Oct. 9, following a change of terms from banking partners. TGOD’s shares went into a tailspin. As of Oct. 22, the company’s stock was trading at CA$1.09 on the Toronto Stock Exchange, down from its September 2018 high of CA$8.25.
Despite TGOD receiving final approval from Health Canada to commence full cultivation operations in the Ancaster facility, the company decided to scale back its production, citing those market forces out of its control. Ancaster’s production goal for 2020 is 12,000 kilograms (~26,455 lbs.) of cannabis (slightly below its full capacity of 17,500 kg (~38,580 lbs.)) as part of the company’s financial restructuring to maintain profitability by Q2 2020, the company said in an Oct. 18 release.
The Valleyfield buildout also is ramping down; the company expects to produce 10,000 kg (~22,046 lbs.)—Phase 1A originally was slated to yield 65,000 kg of cannabis annually, with Phase 1B doubling that capacity. The site’s processing facility also is on hold, and all product from Valleyfield will be processed and packaged at Ancaster. The company estimates that it will need $70 million to $80 million by the end of Q2 2020 to undertake the plan and reach positive operational cash flow, it said in the same release.
“With the current Canadian legal market being smaller than initially anticipated, mainly due to a slow rollout of retail locations in key provinces, we believe that our revised plan will allow TGOD to right size its production to capture the organic segment, while maintaining optionality to quickly accelerate and expand as more retail locations begin to open,” added Athaide in the statement.
Each handful of living soil contains thousands of kilometers of fungal networks.
To avoid being at the mercy of a single market’s shifts and woes, Athaide has an eye on a future where TGOD is in multiple markets. “Our vision is to be the largest organic cannabis and hemp brand globally,” he says. “Our strategy is not to be fully vertically integrated into everything. We are doing the organic cultivation in Canada. We have developed the IP, but as we go international, we’re finding great partners. … Like, for instance, in Poland ... we’re buying third-party organic hemp [for our hemp business] where we’re then drying it, extracting it, creating it into oil.”
Along with Poland, TGOD has operations or agreements with groups in the U.S., Jamaica, Denmark and Mexico. In each of those, “we’ve chosen those parts of the value chain that we believe we can uniquely own and add and do better than anybody else,” Athaide says. “You can be good at a lot of things, you can’t be great at everything. So we’re focusing on those parts that we can be great at and finding great partners for the rest.”
That said, the company’s financial struggles make it difficult for TGOD to focus on international expansion—it doesn’t make sense to spend capital on satellites when the core business isn’t as solidified as it should be. But Cannabis 2.0, Canada’s legal launch of extracted products, has the CEO hopeful. “We have a best-in-class science team that is not only looking at clinical research, but also applied science to help differentiate our products. They have been instrumental in developing our product portfolio for Cannabis 2.0, working closely with other teams on formulations. I am excited about the upcoming launch of our organic teas, infusers and vapes in mid-December,” Athaide says.
Brian MacIver is senior editor for Cannabis Business Times and Cannabis Dispensary magazines.
4 Important Plumbing Considerations for Cannabis Facilities
Departments - Upfront | Quick Tips
What cannabis growers must include in the initial designs for their operations.
A plumbing system is a critical yet costly component of a cannabis facility. That’s why growers can’t overlook proper system design. Each system design should take into account a grower’s specific needs as well as code and regulatory requirements. Proper planning can help growers avoid costly and time-consuming rework. Here are some of the most important plumbing considerations when designing a cannabis facility:
1. Consider and select water reclamation systems early.
One of the common set-ups we come across in cannabis cultivation is a water reclamation system, which filters and reuses wastewater collected by HVAC installations from plant transpiration. Water reclamation systems vary depending on facility size, types of irrigation processes, environmental conditions and compliance and regulations. When designed properly, they can reclaim a very high percentage of wastewater, which, in turn, will reduce water usage, reduce waste and save money. Growers should determine their needs early in the design process, as adding these systems later can be costly from a budget and time standpoint.
2. Understand safety requirements, including eyewash stations and emergency showers.
For cultivators involved in extraction or processing, eyewash stations and/or emergency showers may be necessary, and they are not uncommon in cultivation facilities utilizing fertilizer or another fertigation system. Installing this equipment is typically much more costly and complicated than growers might think. Showers in most residential homes flow at a rate of about 2.5 gallons per minute, while emergency showers typically use 20 gallons per minute and must provide tempered water for 15 minutes. This means water meters, supply mains, water heating equipment and, in some cases, electrical systems, need higher capacities to accommodate the added water supply load.
Also, emergency fixture placement must meet certain standards, such as being located within 55 feet of the hazard, being reachable within 10 seconds and include a path that is free from obstructions, such as steps and doors. It’s important to get this right, as Occupational Safety and Heath Administration (OSHA) penalties can be thousands of dollars.
3. Consider reverse osmosis systems.
Emergency Shower
warut | Adobe Stock
Reverse osmosis (RO) systems are frequently used in cannabis cultivation facilities as a water filtration method. RO water can provide a more consistent way to add nutrients and other additives to a cultivator’s irrigation/fertigation water. But these highly engineered systems are prone to maintenance issues, such as corrosivity and backflow pressure, and other issues due to pipe material choice. Be sure to hire a plumbing engineer with experience in cannabis facility designs to avoid common complications.
4. Install hand sinks—even if they’re not required.
The health department generally requires hand sinks in any sort of food processing environment, and adult-use cannabis cultivation and processing facilities typically fall into this category. Cultivators should check with the health department to understand their facility requirements. Even if hand sinks aren’t required in post-harvest process areas like drying, extraction or in kitchens, growers should consider them as the industry continues to place a higher value on contaminant-free products.
Michael Leavitt is a professional mechanical engineer who has been working with Root Engineers since 2014.
Routine vs. Diagnostic Leaf Tissue Analysis for Cannabis
Features - Nutrient Matters
Tissue testing can tell you if your nutrient program is on target and is one of the best methods for diagnosing nutrient-related disorders.
Foliar tissue analysis is an extremely useful tool for growers managing plant nutrition of cannabis. It falls into two major categories: routine analysis and diagnostic analysis. Routine analysis is for problem prevention, whereas diagnostic analysis is for problem solving.
The routine analysis allows growers to monitor nutrient uptake in the plant during production. This requires a grower to take multiple samples over the entire course of the growing season to identify problems or issues before they become serious concerns.
Growers can use diagnostic analysis to diagnose deficient or toxic nutrients when the plants are displaying negative symptoms. This process helps identify the specific problem and is primarily a corrective tool. Plant tissue analysis is especially useful in determining micronutrient levels in the plant and has a greater level of accuracy than a substrate test using a water-based extraction.
Tissue Analysis Strategies for Cannabis Growers
Routine analysis sampling is a general management tool when the plant is displaying no negative symptoms. The strategy here is to determine the nutrient status of the plant by plotting tissue values over time to ensure that a fertility program is on track.
For example, when growing a cannabis mother stock crop, a grower could collect samples monthly during the time the plant is developing prior to taking cuttings. This would provide valuable information to ensure that plants contain adequate nutrient levels going into the cutting season.
1Source: Bryson, G.M. and H.A. Mills. 2014. Plant analysis handbook IV. Micro-Macro Publ., Athens, GA. 2Source: Landis, H., Hicks, K., Cockson, P., Henry, J.B., Smith, J.T. and Whipker, B.E., 2019. Expanding Leaf Tissue Nutrient Survey Ranges for Greenhouse Cannabidiol-Hemp. Crop, Forage & Turfgrass Management, 5(1).
Another valuable strategy could be to sample the plants just before taking cuttings and then periodically over the cutting season. Typically, with other floriculture species, nutrient levels decline over time as cuttings are harvested. Knowing the status of your stock plants enables growers to modify their fertilization practices to the stock plants during the cutting season to help ensure the cuttings are nutritionally charged when growers take them from the plant.
A third monitoring strategy would be to periodically sample the cuttings taken from the stock plant during the cutting season. This will determine if nutrient levels are being maintained in the stock. This will also verify that the cuttings themselves have adequate nutrients.
Each of these nutritional monitoring strategies requires that growers sample each cultivar separately, as nutrient levels can vary with each one. In addition, if there are differences in the plant’s age or fertilization practices, cultivators should sample those blocks of plants separately.
Cultivators sampling plants for diagnostic analysis should sample them individually. To accurately diagnose a nutritional disorder, growers will need to take samples from the affected plant and/or symptomatic portion of the plant. For example, if the lower leaves display toxicity symptoms, then cultivators should sample those leaves. Because the critical lower deficiency ranges and toxic upper ranges for cannabis are not known at this time, if possible, cultivators should collect a “good” control sample to compare to their problem sample.
Understanding Testing Parameters
Generally, a leaf tissue analysis will include the following macronutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg). (Labs might not include sulfur (S) or may do so at an additional charge.) Tissue analysis also determines the following micronutrient concentrations: boron (B), copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn). Some labs charge extra and require a larger sample amount for molybdenum (Mo) determination, and some will also test for sodium (Na) and chloride (Cl). While Na and Cl are not considered essential for plant production, some fertilizers or source water may contain these nutrients. Cultivators should check with the lab where they are submitting their sample to determine the elements tested and cost.
Sampling Procedure 101
The leaf tissue sample should be representative of the plant or production area being monitored or the problem cultivators wish to analyze. As mentioned above, growers should sample plants of different ages and varieties separately. The process includes:
1. Collecting leaf samples.
For routine analysis, collect the most recently mature leaf. This leaf is typically three to five leaves down from the growing tip (Fig. 1 above). For a representative sample, take 25 to 30 leaves from five to 10 plants (Fig. 2).
For diagnostic analysis, sample plants individually, taking leaves from the affected portion of the plant (Fig. 3). Again, if possible, sample a “good” control so that comparisons can be made.
2. Washing and drying leaves.
If the crop has been overhead watered or micronutrients have been foliar applied, gently wash the leaves in distilled water for 20 seconds to remove any surface contaminants. Dry the leaves with paper towels after washing, prior to packaging them.
3. Packaging leaves for analysis.
Send the samples in a paper bag or manila envelope to the commercial lab authorized to analyze cannabis samples (Fig. 4). Label the bag with your name and address. Contact the lab to verify if there are any special requirements for delivery and documentation under current legislation.
4. Delivering the samples.
Immediately send the samples to a lab certified in your state to conduct leaf-tissue analysis. Try to collect the sample at the beginning of the week so delivery will not be delayed over the weekend. If delivery time to the lab (or to a drying oven) is expected to exceed 12 hours, then it’s best to refrigerate or air-dry the samples.
At this point, nutrient sufficiency ranges for cannabis have not been developed for specific growth stage(s). Bryson and Mills (2014) published nutrient ranges for cannabis (Table 1 above). The database for cannabis nutrient sufficiency ranges is still a work in progress and will be further refined over time as scientific studies determine target nutrient levels over the crop's production cycle as well as critical deficiency and toxicity levels. Refinement of tissue standard ranges for cannabis has been a project being studied by the North Carolina Department of Agriculture and Consumer Services, Agronomic Division and North Carolina State University. Those refined values for cannabis mother stock plants grown in a greenhouse just prior to the stage when cuttings would be harvested are also listed in Table 1.
Conclusion
Leaf tissue analysis provides insight into the nutrient status of a cannabis crop. Tissue analysis results will help growers determine if their fertilizer program is on target by providing current nutrient levels in the plant. In addition, tissue testing is one of the best methods for diagnosing nutrient disorders in cannabis.
Brian Whipker, Paul Cockson, James Turner Smith & Hunter Landis are from the Department of Horticultural Science, North Carolina State University, Raleigh, N.C.
Selling Science: How Research Is Changing the Way the Cannabis Industry Makes Decisions
Features - Business
A look at how cultivators can leverage research to deliver products that meet specific consumer needs.
The terms cannabinoids, terpenoids and flavonoids may not have the same market appeal as indica or sativa. But many industry experts say these secondary metabolites hold more value to cultivators and their customers than common strain names.
In the September 2019 issue of Cannabis Business Times, senior editor Brian MacIver explored some industry myths and how science is changing the way cultivators make strategic decisions (see the cover story “The Science Void”). It’s a topic that CBT also explored during the 2019 Cannabis Conference.
Andrea Sparr-Jaswa, now science editor at CBT and sister publication Cannabis Dispensary, addressed some of these key issues as the moderator for a panel on “Science That Sells: Debunking the Sativa and Indica Myths and Instead Focusing on Terpenoids, Cannabinoids and Flavonoids” at the 2019 event, which featured Jeremy Plumb, director of production science for Prf Cultivar; Jeremy Sackett, founder and chief science officer of Cascadia Labs; and Dr. Dominick Monaco, general manager at GB Sciences Inc.
Here are some highlights from the panel discussion and a glimpse at what’s to come for Cannabis Conference 2020 taking place at Paris Las Vegas April 21-23, 2020. (Note: the following has been edited for length and clarity.)
The “Science That Sells” panel from the 2019 Cannabis Conference. From left to right: Dominick Monaco, Jeremy Sackett, Jeremy Plumb and Andrea Sparr-Jaswa.
Conference Photo by jake Gravbrot
On the Indica vs. Sativa Myth
Plumb: We’ve worked for a long time to try to be more relevant at both Farma and now at my role at Prf Cultivar, to try to … expose the ingredients. But the big issue is this: There’s morphology—like the plant structure. And when we look at narrow and broad leaves, from a plant morphology perspective, we can’t easily correlate that with the kinds of chemistry the plant produces. Really, this morphology was the basis for the original taxonomy.
And it’s gone through many waves. But the reality is, we’re in a new era where we have really distinctive features for phytochemistry on one side of the equation and then distinctive features for the morphological development of the plant on the other. But the giant issue happens when we try to connect the two. And really, [we] can never say that the narrow-leaved sativa plant is producing these focusing and euphoriant effects. That’s really going to come down to the molecules and the inventory and the chemistry in that plant.
Sackett: We’ll never find this perfect archetype of (indica/sativa) in the commercial market now. There’s a third category that’s often used, which is “hybrid.” The problem is … they’re all hybrids. These categories that have become popularized … are a bit unfortunate. You see … we have this moment in history to really change people’s lives with the inventory of phytochemistry, [and] we’re seeing really unfortunate labeling of the ingredients or misrepresentations altogether.
Sparr-Jaswa: Yeah, hybridization in the market and just this constant evolution of perpetuating new genetics—which is incredibly exciting—has completely rendered the [indica/sativa] dichotomy meaningless.
Jolygon | iStockPhoto
On Data Transparency and Targeted Effects
Monaco: If we don’t actually start collecting that [phytochemical] data, we don’t know if it’s actually having effects on therapeutic value for our patients or our customers. There could be a phytol, a sterol, an ester in there that’s causing all of these actual great therapeutic effects. Metabolomics [the study of metabolites’ effects on cells] is definitely where we’re going to find out exactly where the plant and body meet. We always talk about seed-to-sale, right? But we need to talk about seed-to-outcomes … because that’s where we’re going to get all of this actual data … where we’re going to push this forward.
Sackett: And, you know, that outcome at this juncture, as you mentioned, is really patient-based and empowering the patient or adult consumer to really understand what they are consuming. It just takes a little bit of motivation on the retail end. And then, hopefully, empowering the consumer to start tracking their outcome based on these sets of terpene and cannabinoid information that they now have.
Plumb: [Data allows us to] really meet people’s needs and take care of them in a more targeted way. … It always comes back on us to actually report and collect that data in a wholehearted way—to make sure it’s transparent and make sure that it’s not adulterated in any way, and that it’s analyzed correctly. Because, you know, if we start correlating things that aren’t correlatable, then we’re back to square one.
On Navigating Environmental Effects on Phytochemistry
Plumb: The reality is, if you are a producer as I am, you would see an unbelievable range of phytochemistry from one plant as a result of small changes in the environment. So, the kinds of things that contribute to affecting the secondary metabolite profile of drugs the plant is making include: temperature, relative humidity, light intensity, light wavelength, frequency of irrigation, nutrition programs, harvest timing—all sorts of things beyond the genetic material itself.
… So, for people who advocate sun-growing—which I am a huge fan of—we need to absolutely ecologically produce cannabis as … our carbon footprint scales. … But, on the other hand, we really need targeted effects and plants that can produce consistent arrays of phytochemistry in order to meet diverse people’s needs. So, there’s a really important role to play in controlled environment agriculture—in precision agriculture.
Monaco: You know, having cultivated 1.5 million grams every year out of our local Las Vegas facility, I can attest to the fact that it’s very difficult to get batches to be the same. We’ve noticed variance from room to room, time of year. … And having that laboratory data to come and back-end your actual production data to say, “OK, so my VPD went off here or my light shut off here or my CO2 went off and I had to switch out a tank for that day.” ... Knowing those kinds of things and then correlating it with the actual laboratory data is absolutely key if you want to fine-tune your operation.
Jolygon | iStockPhoto
On Nomenclature and Setting Consumer Expectations
Sackett: And speaking to the standardization of testing labs … there are many national organizations working to set those standards. … The general quality practices of any laboratory are consistent to a food laboratory, a pharmaceutical laboratory. But I’ve sat in rooms where [strain] names were changed just because the marketing team thought it would sell better under this name. I think ... what is in that name is really those phytochemicals. Using that profile to navigate both consumer choices as well as those breeding and cultivation choices is super important.
Plumb: We’re still at a moment where there is an incredible amount of genetic diversity. … And we have an opportunity to actually do an honest inventory and start to represent the products more accurately to consumers. So clearly diminishing the relevance of the strain name is a fundamental part of that and really moving forward the chemovar … is really the most important thing I see happening in terms of being more relevant to a mass market.
Sackett: Moving a product forward with those different claims of experience is a little bit early in my opinion. … And, ultimately, a dream that I have is that somebody can walk into a retail shop and be drawn to a terpinolene-dominant section, a caryophyllene-dominant section, a pinene-dominant section and have those grouped together and be able to make better choices.
So, I really think that we’re at a place where it’s just going to take a while, and we have to be humble and use great science and collaborate and be mature as an industry. And recognize what we don’t know and where we have to go to be able to serve the most people at the deepest level.
Jonathan Katz is managing editor for Cannabis Business Times and Cannabis Dispensary magazines.
Legislative Map
Cannabis Business Times’ interactive legislative map is another tool to help cultivators quickly navigate state cannabis laws and find news relevant to their markets. View More